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Nucleic acid purification

DNA and RNA extraction principles, sample handling, purity checks and the limits of a clean spectrophotometric ratio.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
specificationenquiryglossarytroubleshootingcontaminationcontrolsquantificationpurificationbenchesaliquotsblanksabsorbancecarryoverextractionno rt controlstoragetissuedilution

26 articles

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

What a 260/280 ratio does not prove

Read a 260/280 ratio as a protein-contamination hint, and see why the number cannot prove integrity, species identity, or that a later PCR will succeed.

8 October 2026 · 9 min read

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

When to repeat an extraction

Compare repeating the extraction, diluting and retrying the assay, and cleaning the same eluate. Use blanks, labels, yield and debris to choose.

8 October 2026 · 7 min read