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no rt control

Articles tagged no rt control. Tags group methods across clusters so a technique can be found from more than one route.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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3 articles

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

DNase treatment and its limits

Decide when DNase should remove genomic DNA from an RNA prep, and where the enzyme can nick RNA or remain active into reverse transcription.

8 October 2026 · 9 min read

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

gene expression

Intron-spanning primers and genomic DNA

How intron-spanning primers make genomic DNA a different product from cDNA, and when a no-RT control is the only honest check.

8 October 2026 · 9 min read

Gloved hand closing the lid of a benchtop PCR thermal cycler holding a strip of PCR tubes, city lights at dusk behind

pcr amplification

Reverse transcription before PCR

Troubleshoot the reverse-transcription step before PCR: two enzymes, primer class, and a no-RT control for genomic DNA. This is not quantification.

8 October 2026 · 8 min read