immunoassays
Calibrators and what they are traceable to
A calibrator defines the ELISA x-axis. Traceability is a documented chain to a stated reference. Many research standards are assay units only.
8 October 2026 · 9 min read
Tag
elisa
Articles tagged elisa. Tags group methods across clusters so a technique can be found from more than one route.
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12 articles
immunoassays
Comparing ELISA and western blot evidence
ELISA returns a number when the curve holds. A blot returns a band at a mass. Their disagreements point at epitope, matrix, or the hook effect.
8 October 2026 · 10 min read
immunoassays
Dilution series instead of a single guess
Why one antibody or sample dilution is a guess, and how a dilution series or checkerboard finds the window where the blank stays flat.
8 October 2026 · 9 min read
immunoassays
Edge effects on a microplate
Outer wells lose volume and drift in temperature compared with the centre. Compare seals, humidity and layout before you treat an edge well as biology.
8 October 2026 · 11 min read
Pillar · immunoassays
ELISA formats controls and readout
ELISA formats, controls and readout: how direct, sandwich and competitive assays turn a binding event into a number that stays inside the curve.
8 October 2026 · 8 min read
immunoassays
Hook effect and dilute-to-check
Why a very high analyte can make a sandwich ELISA look low, and how a planned dilution series separates a hook effect from a truly low sample.
8 October 2026 · 10 min read
immunoassays
Lot changes and why a control sample matters
How a frozen control sample shows that an antibody or kit lot has shifted, and what to ask when you bridge an old lot to a new one.
8 October 2026 · 9 min read
immunoassays
Reporting an immunoassay so another lab can audit it
An auditable immunoassay file holds lots, the unit, the curve model, raw blanks and excluded wells. A bar on a slide does not replace them.
8 October 2026 · 9 min read
immunoassays
Sandwich direct and competitive ELISA
How to choose a direct, indirect, sandwich or competitive ELISA from analyte size, available epitopes and whether signal should rise or fall.
8 October 2026 · 10 min read
immunoassays
Standard curves that bend
Why immunoassay standard curves are sigmoid, where the usable window sits, and what an early bend or a forced straight line does to a reported value.
8 October 2026 · 9 min read
immunoassays
Troubleshooting a high background
High background means the blank is loud. Separate a dirty blank from a plate that is loud in every well before you subtract and move on.
8 October 2026 · 9 min read
immunoassays
Troubleshooting no signal
A flat research ELISA is a broken chain, from coating to wavelength. Separate a truly blank plate from a signal that merely sits below the curve.
8 October 2026 · 11 min read