chromatography
A glossary of chromatography terms
Use chromatography terms with their real limits: retention, resolution, void, and a peak that is only a detector response.
8 October 2026 · 7 min read
Topic cluster
Chromatography
HPLC and column chromatography concepts for life-science samples, including resin choice and fraction checks.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
specificationenquiryglossarytroubleshootingbuffersfluorescencepeptidesphpurificationblanksmass spectrometrysolventsassayscarryoverstorageaffinitychromatogramchromatography
26 articles
chromatography
Affinity and ion-exchange protein purification
How affinity capture and ion exchange pull a protein from a lysate, and why each collected fraction still needs an identity check.
8 October 2026 · 9 min read
chromatography
Affinity tags and competitive elution
Choose an affinity tag class and a competitive elution that the resin protocol supports, then decide which fractions to keep.
8 October 2026 · 7 min read
chromatography
Buffers that crash out on a column
Spot mobile phases that precipitate on a column: phosphate with organic solvent, high salt, cold beds, and why you filter.
8 October 2026 · 7 min read
chromatography
Carryover between injections
Trace carryover to the needle, a weak wash, or a missing blank, and decide whether the next peak belongs to the previous sample.
8 October 2026 · 7 min read
Pillar · chromatography
Chromatography methods in life-science labs
How life-science chromatography separates molecules on a stationary phase, and what an HPLC or column peak does not prove about purity.
8 October 2026 · 9 min read
chromatography
Cleaning and storing a column
Clean and store a column from its insert: salt and organic washes, then the storage solvent that insert names, not a shared cocktail.
8 October 2026 · 7 min read
chromatography
Column volume flow rate and pressure
Judge whether a column flow is safe: column volume, linear velocity, residence time, and backpressure as a health signal.
8 October 2026 · 8 min read
chromatography
Comparing HPLC and open-column work
Decide when HPLC pressure and resolution are worth it, and when a gravity column and larger fractions are the honest tool.
8 October 2026 · 7 min read
chromatography
Detectors UV fluorescence and mass spec
Choose UV, fluorescence, or mass spectrometry by what each detector can report, and what it still cannot identify alone.
8 October 2026 · 7 min read
chromatography
Documenting a method someone else can run
Write a chromatography method another person can run: column, lot, phases, gradient, flow, temperature, injection, detection, and dwell.
8 October 2026 · 6 min read
chromatography
Fraction collection and how to assay fractions
Decide whether a fraction is worth pooling: collect by volume or peak, then assay activity, a gel, or absorbance before you combine.
8 October 2026 · 7 min read