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ph

Articles tagged ph. Tags group methods across clusters so a technique can be found from more than one route.

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6 articles

pH electrode in a beaker of buffer while a gloved hand adds drops from a dropper bottle

buffers and water

Autoclaving a buffer can shift pH

Autoclave a buffer only when the composition allows it, then recheck pH at the recipe temperature, because Tris, sugars, and bicarbonate do not come back

8 October 2026 · 9 min read

pH electrode in a beaker of buffer while a gloved hand adds drops from a dropper bottle

buffers and water

CO2 and why pH drifts in a carboy

When carboy pH falls overnight, follow dissolved carbon dioxide and buffer capacity first, then decide whether the electrode is the failed control.

8 October 2026 · 8 min read

pH electrode in a beaker of buffer while a gloved hand adds drops from a dropper bottle

chromatography

Ion exchange pH and salt

Set ion-exchange pH against the protein charge and plan a salt gradient that binds the target and releases it on purpose.

8 October 2026 · 8 min read

Gloved hand weighing white powder with a spatula inside an analytical balance draft shield

buffers and water

pH electrodes storage and calibration

Store the glass electrode in its storage solution, calibrate with fresh buffers that bracket the target, and treat a tired slope as a failed control.

8 October 2026 · 9 min read

pH electrode in a beaker of buffer while a gloved hand adds drops from a dropper bottle

buffers and water

Preparing a buffer and checking pH

Prepare a buffer at a stated pH and temperature, record the salt form, and treat a drifting electrode or a bad slope as a failed control.

8 October 2026 · 11 min read

pH electrode in a beaker of buffer while a gloved hand adds drops from a dropper bottle

buffers and water

Temperature compensation on a pH meter

If Tris pH still moves while ATC is on, the meter corrected the electrode slope. The buffer's own chemistry is a separate temperature effect.

8 October 2026 · 8 min read