dna sequencing
A glossary of sequencing terms
Working definitions of read, coverage, depth, MAPQ, Phred, VCF, BAM and the related words, each tied to the mistake that word prevents.
8 October 2026 · 9 min read
Tag
sequencing
Articles tagged sequencing. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
18 articles
genome editing
Checking whether a genome edit worked
How PCR, sequencing and protein checks show whether a genome edit is present, clonal and on target, and which result is still only a hint.
8 October 2026 · 8 min read
dna sequencing
Choosing read length and paired ends
Choose read length and paired or single ends from the sequence you must place. Longer short reads help repeats only a little; mates rescue mapping.
8 October 2026 · 7 min read
research sourcing
Commissioning a sequencing or proteomics study
Commission sequencing or proteomics by locking the question, the reference database, sample QC and the deliverable before a library or a digest is made.
8 October 2026 · 10 min read
dna sequencing
Data retention and who holds the raw files
Who keeps FASTQ, BAM and VCF, for how long, and what to do before a download link closes. Retention is a custody decision, not a file habit.
8 October 2026 · 8 min read
dna sequencing
From reads to a result a biologist can question
QC, trim, align, deduplicate, call, annotate, filter: the questions a biologist can still ask, and why a PDF gene list is not the data.
8 October 2026 · 8 min read
dna sequencing
Long-read sequencing for structural variants
How to judge whether a long read can span an insertion, deletion, inversion or repeat that short reads only split or fail to cross.
8 October 2026 · 9 min read
Pillar · dna sequencing
Next-generation sequencing from library to reads
How a sequencing library becomes reads: adapters, flow cells, quality scores and the checks that stop a bad library from wasting a run.
8 October 2026 · 8 min read
genome editing
Off-target checks and their limits
In silico lists, GUIDE-seq, CIRCLE-seq, nominated amplicons and genome sequencing each answer a narrower off-target question than the word clean implies.
8 October 2026 · 10 min read
research sourcing
Pilot samples before a large cohort
Separate what a pilot may decide, such as yield, library QC, and logistics, from the biological effect only a designed cohort can support.
8 October 2026 · 9 min read
genome editing
Questions before an editing-validation enquiry
An editing-validation enquiry needs cell type, delivery class, guide sequences, genome build, locus, sample count and the readout you want. A gene name alone is not enough.
8 October 2026 · 7 min read
dna sequencing
Sample identity mix-ups and plate maps
A swapped column or a mismatched index ruins identity before chemistry starts. How plate maps, barcodes and fingerprints catch the swap.
8 October 2026 · 8 min read