cytometry and microscopy
Controls for autofluorescence
How to separate autofluorescence from a real fluorophore using matched unstained cells, the same treatment, and the right detector.
8 October 2026 · 7 min read
Tag
fluorescence
Articles tagged fluorescence. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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7 articles
chromatography
Detectors UV fluorescence and mass spec
Choose UV, fluorescence, or mass spectrometry by what each detector can report, and what it still cannot identify alone.
8 October 2026 · 7 min read
cytometry and microscopy
Fluorescence microscopy without the myths
How a fluorescence microscope selects excitation and emission, and which bright pictures still fail as evidence of a labelled molecule.
8 October 2026 · 10 min read
Pillar · cytometry and microscopy
How a flow cytometer reads a cell
How a cytometer focuses cells into a laser, turns scatter and fluorescence into pulses, and why a gate is an argument rather than a fact.
8 October 2026 · 8 min read
labware
Plate colours clear white and black
How to trace a weak or noisy plate reading to well colour: clear for absorbance, white for luminescence, and black for fluorescence.
8 October 2026 · 7 min read
immunoassays
Plate readers absorbance and fluorescence
Absorbance, fluorescence and luminescence are different reads. A wrong wavelength or a clear plate in a fluorescence assay looks like a failed ELISA.
8 October 2026 · 9 min read
cytometry and microscopy
Troubleshooting a dim microscope image
How to check shutter, filter cube, objective, correction collar, focus and exposure before calling a dim field a negative result.
8 October 2026 · 7 min read