Tag

peptides

Articles tagged peptides. Tags group methods across clusters so a technique can be found from more than one route.

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6 articles

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

Pillar · proteomics

Bottom-up proteomics in plain language

Bottom-up proteomics in plain language: proteins are digested to peptides, a mass spectrometer fragments them, and a database search names candidates.

8 October 2026 · 7 min read

proteomics

Missed cleavages and ragged ends

How an internal lysine or arginine differs from a ragged peptide end, what a high missed-cleavage rate says about the digest, and how search settings affect the

8 October 2026 · 10 min read

proteomics

Peptide fractionation overview

Single-shot runs versus offline peptide fractionation: depth, instrument time, sample use, concatenation and the losses that come with extra fractions.

8 October 2026 · 8 min read

proteomics

Preparing peptides for mass spectrometry

How laboratories prepare peptides for mass spectrometry: reduction, alkylation, digestion, cleanup, and the clues that a digest failed.

8 October 2026 · 7 min read

proteomics

Reduction alkylation and trypsin

Why proteins are reduced and alkylated before trypsin in bottom-up proteomics, and how incomplete cysteine chemistry changes which peptides a search can name.

8 October 2026 · 11 min read

chromatography

Reversed phase for peptides

Diagnose ruined peptide reversed-phase peaks: hydrophobic retention, acetonitrile gradients, and a sample solvent that is too strong.

8 October 2026 · 7 min read