chromatography
Gradients versus isocratic runs
Choose an isocratic hold or a solvent gradient from the resolution you need, then budget dwell volume and column equilibration.
8 October 2026 · 7 min read
Topic cluster
Chromatography
HPLC and column chromatography concepts for life-science samples, including resin choice and fraction checks.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
specificationenquiryglossarytroubleshootingbuffersfluorescencepeptidesphpurificationblanksmass spectrometrysolventsassayscarryoverstorageaffinitychromatogramchromatography
26 articles
chromatography
Imidazole concentration and nickel resin
Set imidazole for a nickel resin from the manufacturer range: a low-millimolar wash and a higher-millimolar elution, not a guess.
8 October 2026 · 7 min read
chromatography
Ion exchange pH and salt
Set ion-exchange pH against the protein charge and plan a salt gradient that binds the target and releases it on purpose.
8 October 2026 · 8 min read
chromatography
Normal phase is a different solvent logic
Choose normal phase only when its polar stationary phase and non-polar mobile phase match the analyte better than reversed phase.
8 October 2026 · 7 min read
chromatography
Questions before a column enquiry
Write a column enquiry that names chemistry, particle size, dimensions, pressure rating, pH window, matrix, and analytical or prep goal.
8 October 2026 · 7 min read
chromatography
Reversed phase for peptides
Diagnose ruined peptide reversed-phase peaks: hydrophobic retention, acetonitrile gradients, and a sample solvent that is too strong.
8 October 2026 · 7 min read
chromatography
Sample solvents that wreck a peak shape
Name the sample-solvent failures that split, front, or break through a peak, and decide whether to dilute before the next injection.
8 October 2026 · 7 min read
chromatography
Scaling a prep from a scout column
Scale a prep from a scout column by holding bed height and linear velocity, scaling load with volume, and not copying pressure.
8 October 2026 · 7 min read
chromatography
Size-exclusion chromatography concepts
Plan a size-exclusion run that separates aggregates from monomer, and decide what sample volume that bed can honestly accept.
8 October 2026 · 9 min read
chromatography
Solvent quality and ghost peaks
Separate a ghost peak from a sample peak with a blank gradient, and judge water, solvent, and plastic leachables before you integrate.
8 October 2026 · 7 min read
chromatography
Standards and retention time windows
Set a retention window from a standard on this method, and widen or stop when temperature, column age, or mobile phase moves it.
8 October 2026 · 7 min read
chromatography
Troubleshooting a flat chromatogram
Work through a flat chromatogram by checking the lamp, wavelength, injection, leak, flow-through, and the integrator scale.
8 October 2026 · 7 min read