Tag

assays

Articles tagged assays. Tags group methods across clusters so a technique can be found from more than one route.

cold chain biosafety crispr specification sequencing proteomics enquiry glossary troubleshooting cloning microbiology buffers freezers pipetting calibration elisa cell culture glassware

3 articles

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

Enzymes lose activity before they look degraded

An enzyme can lose activity while the Coomassie band remains. Oxidation, carryover, a missing cofactor or partial unfolding need an activity assay.

8 October 2026 · 7 min read

Gloved hand placing a vial into the autosampler of a modular HPLC system with solvent bottles on top

chromatography

Fraction collection and how to assay fractions

Decide whether a fraction is worth pooling: collect by volume or peak, then assay activity, a gel, or absorbance before you combine.

8 October 2026 · 7 min read

Wall-mounted water purification unit dispensing ultrapure water into a glass flask

buffers and water

Troubleshooting an assay that worked last month

Choose the first variable to test when an assay that worked last month now fails: water, pH at the temperature of use, the salt hydrate, the electrode, or the

8 October 2026 · 9 min read