Pillar · proteomics
Bottom-up proteomics in plain language
Bottom-up proteomics in plain language: proteins are digested to peptides, a mass spectrometer fragments them, and a database search names candidates.
8 October 2026 · 7 min read
Tag
mass spectrometry
Articles tagged mass spectrometry. Tags group methods across clusters so a technique can be found from more than one route.
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4 articles
chromatography
Detectors UV fluorescence and mass spec
Choose UV, fluorescence, or mass spectrometry by what each detector can report, and what it still cannot identify alone.
8 October 2026 · 7 min read
protein biochemistry
Detergents that ruin a later mass spec step
How SDS, Triton, NP-40 and Tween spoil a mass spectrum, which removal class to consider, and what to tell the person who will run it.
8 October 2026 · 9 min read
proteomics
Reduction alkylation and trypsin
Why proteins are reduced and alkylated before trypsin in bottom-up proteomics, and how incomplete cysteine chemistry changes which peptides a search can name.
8 October 2026 · 11 min read