dna sequencing
A glossary of sequencing terms
Working definitions of read, coverage, depth, MAPQ, Phred, VCF, BAM and the related words, each tied to the mistake that word prevents.
8 October 2026 · 9 min read
Tag
bioinformatics
Articles tagged bioinformatics. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
3 articles
research sourcing
Bioinformatics analysis as a separate line
Treat alignment, quantification, and statistics as their own line, and name the reference, the contrasts, and the file types before reads are produced.
8 October 2026 · 9 min read
dna sequencing
From reads to a result a biologist can question
QC, trim, align, deduplicate, call, annotate, filter: the questions a biologist can still ask, and why a PDF gene list is not the data.
8 October 2026 · 8 min read