proteomics
A protein ID is not a proof of function
A proteomics identification is a sequence match under a false discovery rate. Function still needs activity, phenotype, localisation or a perturbation, plus
8 October 2026 · 8 min read
Tag
evidence
Articles tagged evidence. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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4 articles
immunoassays
Comparing ELISA and western blot evidence
ELISA returns a number when the curve holds. A blot returns a band at a mass. Their disagreements point at epitope, matrix, or the hook effect.
8 October 2026 · 10 min read
genome editing
Comparing knockout and knockdown evidence
A knockout is a permanent DNA allele backed by orthogonal evidence. A knockdown only lowers the transcript. A shift in Cq does not establish a knockout.
8 October 2026 · 7 min read
proteomics
Post-translational modifications need extra evidence
Why a variable modification explains a mass shift without proving biology: localisation, neutral loss, search-space cost and which shifts are often tube
8 October 2026 · 7 min read