cytometry and microscopy
Comparing flow and imaging for the same question
How to decide whether a question needs a cytometer count or a microscope location, and what neither result is allowed to claim.
8 October 2026 · 7 min read
Tag
flow cytometry
Articles tagged flow cytometry. Tags group methods across clusters so a technique can be found from more than one route.
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5 articles
cytometry and microscopy
Forward scatter side scatter and a gate
How to read forward and side scatter, draw a gate you can defend, and keep that gate with the file before naming a population.
8 October 2026 · 9 min read
Pillar · cytometry and microscopy
How a flow cytometer reads a cell
How a cytometer focuses cells into a laser, turns scatter and fluorescence into pulses, and why a gate is an argument rather than a fact.
8 October 2026 · 8 min read
cytometry and microscopy
Single-stain controls
How to build single-stain controls that are bright enough for compensation, and when to branch to cells, beads, or a fresh tube.
8 October 2026 · 8 min read
cytometry and microscopy
Viability dyes and dead-cell artefacts
How dead cells bind antibody nonspecifically, and which amine-reactive or DNA-dye class fits a fixed or unfixed staining workflow.
8 October 2026 · 7 min read