cytometry and microscopy
Compensation is about overlap not brightness
Why compensation subtracts spectral overlap measured from single stains, and why sliding brightness cannot repair a spillover matrix.
8 October 2026 · 7 min read
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fluorophore
Articles tagged fluorophore. Tags group methods across clusters so a technique can be found from more than one route.
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3 articles
cytometry and microscopy
Panel design starts with the biology
Which biology, antigen density and spillover rules to name before you assign fluorophores or request a cytometer configuration.
8 October 2026 · 7 min read
cytometry and microscopy
Spectral overlap in a simple three-colour panel
What emission overlap, spillover, spreading and a filter window mean when three fluorophores share one cytometer layout.
8 October 2026 · 7 min read