dna sequencing
Adapters indexes and barcode hopping
Separate adapter sequence from i5 and i7 indexes, and see why unique dual indexes resist barcode hopping better than one shared index.
8 October 2026 · 8 min read
Tag
indexes
Articles tagged indexes. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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2 articles
dna sequencing
Index collisions in multiplexed pools
Same index twice, indexes one edit apart, and sheets written as the reverse complement. How those collisions misassign reads in a multiplexed pool.
8 October 2026 · 8 min read