molecular cloning
Choosing restriction sites that actually cut
Why a restriction site on a plasmid map may not cut the DNA in your tube, and how methylation, star activity and buffer choice decide the digest.
8 October 2026 · 9 min read
Tag
methylation
Articles tagged methylation. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
2 articles
molecular cloning
Dephosphorylation methylation and cloning strain choice
Choose one fix that matches the evidence: vector phosphatase, a dam/dcm strain when the card says so, or a strain for ccdB or unstable inserts.
8 October 2026 · 7 min read