dna sequencing
Controls and spike-ins for a sequencing lane
Which controls belong on a DNA sequencing lane, why an RNA spike-in does not, and what a failed PhiX-style or known-variant control should stop.
8 October 2026 · 9 min read
Tag
ngs
Articles tagged ngs. Tags group methods across clusters so a technique can be found from more than one route.
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4 articles
Pillar · dna sequencing
Next-generation sequencing from library to reads
How a sequencing library becomes reads: adapters, flow cells, quality scores and the checks that stop a bad library from wasting a run.
8 October 2026 · 8 min read
dna sequencing
Questions for a Sanger versus NGS decision
Questions that separate a Sanger trace from a short-read pool: one amplicon, a mixture, a haplotype, a plasmid junction. Turnaround is asked, not promised.
8 October 2026 · 8 min read
dna sequencing
Troubleshooting a failed library quantification
A library number is not a molarity until the assay is named. How dye, adapter qPCR and a fragment trace disagree, and which disagreement matters.
8 October 2026 · 7 min read