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primers

Articles tagged primers. Tags group methods across clusters so a technique can be found from more than one route.

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2 articles

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

dna sequencing

Amplicon sequencing for a defined region

Use amplicon sequencing only for the region the primers define, and see why depth, chimeras and primer dropout are not a genome.

8 October 2026 · 10 min read

Gloved hand closing the lid of a benchtop PCR thermal cycler holding a strip of PCR tubes, city lights at dusk behind

gene expression

Standard curves and primer efficiency

How a standard curve turns primer slope into efficiency, and how R-squared, dynamic range, no-template controls and primer dimers decide if the line is usable.

8 October 2026 · 10 min read