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restriction digest

Articles tagged restriction digest. Tags group methods across clusters so a technique can be found from more than one route.

1 article

Gloved hand pipetting into a tube near a cold block and agar plates with colonies, gel image on a monitor

molecular cloning

Choosing restriction sites that actually cut

Why a restriction site on a plasmid map may not cut the DNA in your tube, and how methylation, star activity and buffer choice decide the digest.

8 October 2026 · 9 min read