
molecular cloning
Choosing restriction sites that actually cut
Why a restriction site on a plasmid map may not cut the DNA in your tube, and how methylation, star activity and buffer choice decide the digest.
8 October 2026 · 9 min read
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Articles tagged restriction digest. Tags group methods across clusters so a technique can be found from more than one route.
1 article

molecular cloning
Why a restriction site on a plasmid map may not cut the DNA in your tube, and how methylation, star activity and buffer choice decide the digest.
8 October 2026 · 9 min read