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t7e1

Articles tagged t7e1. Tags group methods across clusters so a technique can be found from more than one route.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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2 articles

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

genome editing

T7 endonuclease and amplicon sequencing

T7 endonuclease cuts heteroduplexes from mixed alleles. Amplicon sequencing names bases. A uniform mutant clone can look negative on the gel assay.

8 October 2026 · 9 min read

Visualisation of a protein complex binding a DNA double helix, representing genome editing

genome editing

Troubleshooting no cutting on a surveyor assay

A Surveyor or T7E1 gel with no cut can mean identical alleles, a skipped anneal, a dead enzyme, a failed PCR, no real cut, or bands the gel cannot separate.

8 October 2026 · 7 min read