pcr amplification
Choosing an annealing temperature
Why annealing temperature is not the Tm printed on a tube, and how salt, magnesium, additives, and a short gradient locate the real window.
8 October 2026 · 8 min read
Tag
thermal cycling
Articles tagged thermal cycling. Tags group methods across clusters so a technique can be found from more than one route.
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2 articles
pcr amplification
Extension time and amplicon length
Match extension time to amplicon length, see why a short hold leaves partial products, and why a final extension cannot repair that miss.
8 October 2026 · 8 min read