genome editing
Guide RNA design is a hypothesis
A guide RNA is a hypothesis about spacer, PAM and score. This page says what those numbers can rank, and what only the edited alleles can prove.
8 October 2026 · 10 min read
Topic cluster
Genome editing
Research explainers of CRISPR and related editing concepts, including guides, repair outcomes and how edits are checked.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
biosafetycrisprsequencingenquiryglossarycontrolsvalidationrecordsevidenceampliconcas9clonesfastqethicsguide rnaknockoutt7e1bacteria
26 articles
genome editing
Homology-directed repair versus end joining
End joining, microhomology repair and homology-directed repair compete after a cut. Cell cycle and donor presence decide which outcome is plausible.
8 October 2026 · 8 min read
Pillar · genome editing
How CRISPR-Cas9 editing works in research
How a guide RNA directs Cas9 to a research target, how cells repair the cut, and why an off-target risk is part of the experimental design.
8 October 2026 · 8 min read
genome editing
Indels frameshifts and protein loss
An indel, a frameshift and a missing protein are three claims. Nonsense-mediated decay, exon skipping and extra gene copies keep them apart.
8 October 2026 · 9 min read
genome editing
Mosaicism in edited embryos as a concept
If a nuclease stays active across cleavage divisions, different cells can carry different alleles. One biopsy reports those cells, not the whole organism.
8 October 2026 · 8 min read
genome editing
Off-target checks and their limits
In silico lists, GUIDE-seq, CIRCLE-seq, nominated amplicons and genome sequencing each answer a narrower off-target question than the word clean implies.
8 October 2026 · 10 min read
genome editing
PAM sequences and why the genome context matters
SpCas9 NGG, SaCas9 NNGRRT and Cas12a TTTV are enzyme motifs beside the spacer. A SNP or closed chromatin can remove a site the reference browser still shows.
8 October 2026 · 7 min read
genome editing
Prime editing in plain language
Prime editing writes a short new sequence from a pegRNA, using a nick and reverse transcriptase, without a double-stranded DNA donor.
8 October 2026 · 10 min read
genome editing
Questions before an editing-validation enquiry
An editing-validation enquiry needs cell type, delivery class, guide sequences, genome build, locus, sample count and the readout you want. A gene name alone is
8 October 2026 · 7 min read
genome editing
Recording guides so an experiment can be repeated
Record the spacer 5-prime to 3-prime, the enzyme, the PAM, the assembly, coordinates, strand, modifications, plasmid map and lot so someone else can reorder the
8 October 2026 · 7 min read
genome editing
Selecting edited clones without fooling yourself
Resistance and a fluorescent marker select for a cassette or a transfection, not for a sequenced allele. This page names the traps in clone picking.
8 October 2026 · 8 min read
genome editing
T7 endonuclease and amplicon sequencing
T7 endonuclease cuts heteroduplexes from mixed alleles. Amplicon sequencing names bases. A uniform mutant clone can look negative on the gel assay.
8 October 2026 · 9 min read