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validation

Articles tagged validation. Tags group methods across clusters so a technique can be found from more than one route.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware

7 articles

Visualisation of a protein complex binding a DNA double helix, representing genome editing

genome editing

Checking whether a genome edit worked

How PCR, sequencing and protein checks show whether a genome edit is present, clonal and on target, and which result is still only a hint.

8 October 2026 · 8 min read

Gloved hand holding a stained tissue slide beside a rack of stained microscope slides

immunoassays

Choosing and checking a primary antibody

How to choose and check a primary antibody: host, clonality, application claims, and controls that show the signal is the intended target.

8 October 2026 · 7 min read

Visualisation of a protein complex binding a DNA double helix, representing genome editing

genome editing

Controls for a CRISPR experiment

Place a non-targeting guide, an untransfected well, a known cutter, a second guide and a parental identity check before you treat a fluorescent marker as an edit.

8 October 2026 · 9 min read

Visualisation of a protein complex binding a DNA double helix, representing genome editing

genome editing

Questions before an editing-validation enquiry

An editing-validation enquiry needs cell type, delivery class, guide sequences, genome build, locus, sample count and the readout you want. A gene name alone is not enough.

8 October 2026 · 7 min read

Visualisation of a protein complex binding a DNA double helix, representing genome editing

genome editing

Troubleshooting no cutting on a surveyor assay

A Surveyor or T7E1 gel with no cut can mean identical alleles, a skipped anneal, a dead enzyme, a failed PCR, no real cut, or bands the gel cannot separate.

8 October 2026 · 7 min read

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

genome editing

What sequence data to request after an edit

After an edit, request coordinates, the genome build, FASTQ files, an allele table, coverage and controls. A PDF of the on-target site is not the dataset.

8 October 2026 · 8 min read