genome editing
Homology-directed repair versus end joining
End joining, microhomology repair and homology-directed repair compete after a cut. Cell cycle and donor presence decide which outcome is plausible.
8 October 2026 · 8 min read
Tag
crispr
Articles tagged crispr. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
19 articles
Pillar · genome editing
How CRISPR-Cas9 editing works in research
How a guide RNA directs Cas9 to a research target, how cells repair the cut, and why an off-target risk is part of the experimental design.
8 October 2026 · 8 min read
genome editing
Indels frameshifts and protein loss
An indel, a frameshift and a missing protein are three claims. Nonsense-mediated decay, exon skipping and extra gene copies keep them apart.
8 October 2026 · 9 min read
genome editing
Off-target checks and their limits
In silico lists, GUIDE-seq, CIRCLE-seq, nominated amplicons and genome sequencing each answer a narrower off-target question than the word clean implies.
8 October 2026 · 10 min read
genome editing
Prime editing in plain language
Prime editing writes a short new sequence from a pegRNA, using a nick and reverse transcriptase, without a double-stranded DNA donor.
8 October 2026 · 10 min read
genome editing
Selecting edited clones without fooling yourself
Resistance and a fluorescent marker select for a cassette or a transfection, not for a sequenced allele. This page names the traps in clone picking.
8 October 2026 · 8 min read
genome editing
T7 endonuclease and amplicon sequencing
T7 endonuclease cuts heteroduplexes from mixed alleles. Amplicon sequencing names bases. A uniform mutant clone can look negative on the gel assay.
8 October 2026 · 9 min read