protein biochemistry
His-tags and why a band is not pure protein
A His-tag elution can look like one band and still be a mixture. IMAC is a capture step, and a second method is what supports a purity claim.
8 October 2026 · 6 min read
Tag
his tag
Articles tagged his tag. Tags group methods across clusters so a technique can be found from more than one route.
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2 articles
chromatography
Imidazole concentration and nickel resin
Set imidazole for a nickel resin from the manufacturer range: a low-millimolar wash and a higher-millimolar elution, not a guess.
8 October 2026 · 7 min read