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hybridisation

Articles tagged hybridisation. Tags group methods across clusters so a technique can be found from more than one route.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware

2 articles

pH electrode in a beaker of buffer while a gloved hand adds drops from a dropper bottle

buffers and water

Ionic strength is not the same as pH

During a hybridisation wash, compute ionic strength from the ions you added. A matched pH can still hide a salt change that moves stringency.

8 October 2026 · 7 min read

Agarose gel with glowing DNA bands on a UV transilluminator under an orange safety shield

gels and blotting

Southern blot concepts for DNA

Understand a Southern blot as digest, separation, denaturation, transfer and a probe washed at a stringency that matches the question. Not a diagnostic test.

8 October 2026 · 8 min read