gels and blotting
Gel extraction of a DNA band
Compare a cut DNA band with a column cleanup: UV damage, carryover that blocks ligation, lower yield, and when not to slice.
8 October 2026 · 7 min read
Tag
ligation
Articles tagged ligation. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
4 articles
dna sequencing
Library prep is where most runs are won
See why fragmentation, ligation and extra PCR cycles decide a sequencing run, and which branch to take when library yield is low.
8 October 2026 · 10 min read
molecular cloning
Phosphatase treatment and self-ligation
When to strip 5-prime phosphates from a cut vector so it cannot recircularise, why the insert must keep its phosphates, and how leftover background looks.
8 October 2026 · 8 min read
molecular cloning
Restriction ligation and Gibson assembly
How restriction ligation and Gibson assembly join DNA ends, where each method fails, and what colony screening can and cannot confirm.
8 October 2026 · 8 min read