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loading control

Articles tagged loading control. Tags group methods across clusters so a technique can be found from more than one route.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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2 articles

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

Loading controls are for blots not every gel

Actin, GAPDH and tubulin support a blot loading argument. A purification gel is judged by the band pattern and the total protein you loaded.

8 October 2026 · 6 min read

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

gels and blotting

Loading controls on a western blot

Pick a western loading control for the comparison you are making. A housekeeping band can move, and a total-protein stain is the other argument.

8 October 2026 · 8 min read