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primer dimer

Articles tagged primer dimer. Tags group methods across clusters so a technique can be found from more than one route.

2 articles

Gloved hand closing the lid of a benchtop PCR thermal cycler holding a strip of PCR tubes, city lights at dusk behind

pcr amplification

Hot-start polymerases and primer-dimers

How a heat-released hot-start block limits extension during setup, and how a short no-template product on a gel is read as primer-dimer.

8 October 2026 · 9 min read

Researcher viewing gene expression heatmaps and genomic tracks on two monitors at night

gene expression

Inhibition and weird Cq values

How late, early or impossible Cq values point to inhibition, primer-dimer or a failed control, and which check separates those causes.

8 October 2026 · 9 min read