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antibody

Articles tagged antibody. Tags group methods across clusters so a technique can be found from more than one route.

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5 articles

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Cross-reactivity and related proteins

How to check whether an antibody binds a paralogue or an orthologue, using sequence context and a related-protein lane that should stay quiet.

8 October 2026 · 9 min read

Gloved hand holding a stained tissue slide beside a rack of stained microscope slides

immunoassays

Immunohistochemistry is a different matrix

Why an antibody that works in an ELISA can fail on fixed tissue, and which controls make an immunohistochemistry result a research observation.

8 October 2026 · 8 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Isotype controls are not a full answer

What an isotype control actually measures, which sticky artefacts it can rule out, and why it cannot replace a knockout or a secondary-only well.

8 October 2026 · 8 min read

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

gels and blotting

Western blot from gel to membrane

How a protein moves from an SDS gel onto a membrane, how antibodies create a band, and which controls separate transfer failure from a real signal.

8 October 2026 · 8 min read