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colony pcr

Articles tagged colony pcr. Tags group methods across clusters so a technique can be found from more than one route.

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2 articles

Gloved hand streaking an agar plate with an inoculating loop beside a stack of cultured plates

pcr amplification

Colony PCR for screening clones

Screen colonies with flanking or insert primers, a lysis step, an empty-vector colony, and a no-template control. A band is not a sequence.

8 October 2026 · 9 min read

Gloved hand streaking an agar plate with an inoculating loop beside a stack of cultured plates

molecular cloning

Colony PCR versus diagnostic digest

When to triage colonies by PCR and when a miniprep digest is worth it, including false positives from agar DNA, orientation, and why neither is a sequence.

8 October 2026 · 8 min read