microbiology
Antibiotic selection and blue-white screening
How antibiotic plates keep a plasmid in a cloning strain, and why a white colony on X-gal is a candidate rather than proof of the insert.
8 October 2026 · 9 min read
Tag
plasmids
Articles tagged plasmids. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
3 articles
pcr amplification
Colony PCR for screening clones
Screen colonies with flanking or insert primers, a lysis step, an empty-vector colony, and a no-template control. A band is not a sequence.
8 October 2026 · 9 min read
microbiology
Plasmid stability in a liquid culture
What segregational stability means in liquid culture, and why a cloudy cloning-strain flask can still give you no plasmid.
8 October 2026 · 7 min read