gene expression
A heatmap is a picture not a conclusion
How clustering, colour scale and row scaling change a gene-expression heatmap, and why the differential table and the contrast remain the result.
8 October 2026 · 8 min read
Tag
rna seq
Articles tagged rna seq. Tags group methods across clusters so a technique can be found from more than one route.
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8 articles
gene expression
Batch effects in an expression study
What a batch effect is in an expression study, how library date, operator, sequencer or kit lot can dominate a PCA, and what a correction model can adjust.
8 October 2026 · 8 min read
gene expression
Biological versus technical replicates
How to tell a biological replicate from a technical one, and which unit of inference actually supports a treatment claim in an expression study.
8 October 2026 · 8 min read
gene expression
Comparing RT-qPCR and RNA-seq on the same question
Why RT-qPCR and RNA-seq can disagree on one gene, and when that disagreement means they measured different molecules rather than a failed assay.
8 October 2026 · 8 min read
Pillar · gene expression
From cells to a gene expression result
How a laboratory goes from cells to a gene expression result, and how RNA quality decides between a focused RT-qPCR assay and RNA-seq.
8 October 2026 · 7 min read
gene expression
Pathway lists and the multiple-testing problem
How testing thousands of genes creates a multiple-testing problem, and why a pathway table built on an already chosen list is not yet a mechanism.
8 October 2026 · 10 min read
gene expression
RNA-seq library types polyA and ribodepletion
How poly(A) selection and ribosomal depletion keep different RNA molecules, and when degraded, bacterial or non-polyadenylated samples need the broader library.
8 October 2026 · 8 min read
gene expression
Single-cell RNA-seq in outline
What single-cell RNA-seq adds beyond a bulk average: droplets or wells, empty droplets, doublets, UMIs, sparse counts, and clustering as a parameter.
8 October 2026 · 7 min read