gene expression
Choosing reference genes instead of hoping
How to choose reference genes for this experiment by testing stability, instead of trusting a housekeeping name such as GAPDH or ACTB from another study.
8 October 2026 · 9 min read
Tag
rt qpcr
Articles tagged rt qpcr. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
6 articles
gene expression
Comparing RT-qPCR and RNA-seq on the same question
Why RT-qPCR and RNA-seq can disagree on one gene, and when that disagreement means they measured different molecules rather than a failed assay.
8 October 2026 · 8 min read
gene expression
Delta-delta Cq and what it assumes
What delta-delta Cq assumes about efficiency, the calibrator and reference genes, and why the fold change is a relative comparison under those conditions.
8 October 2026 · 8 min read
Pillar · gene expression
From cells to a gene expression result
How a laboratory goes from cells to a gene expression result, and how RNA quality decides between a focused RT-qPCR assay and RNA-seq.
8 October 2026 · 7 min read
gene expression
RT-qPCR for relative expression
How RT-qPCR for relative expression compares transcripts, using reference genes, efficiency checks and controls that keep a fold change honest.
8 October 2026 · 8 min read
gene expression
The MIQE idea in everyday language
What a qPCR report should disclose so another scientist can judge the number: sample handling, reverse transcription, primers, efficiency, reference genes and
8 October 2026 · 10 min read