microbiology
Plasmid stability in a liquid culture
What segregational stability means in liquid culture, and why a cloudy cloning-strain flask can still give you no plasmid.
8 October 2026 · 7 min read
Tag
selection
Articles tagged selection. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
3 articles
microbiology
Troubleshooting a lawn of colonies
A lawn on selective agar usually means the antibiotic failed or far too many cells were plated. Separate those causes before you treat the plate as a success.
8 October 2026 · 10 min read
molecular cloning
Troubleshooting plates with no colonies
An empty plate is a place to define competent cells, ligation, selection, and transformation efficiency, then test the failure that actually fits.
8 October 2026 · 7 min read