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western blot

Articles tagged western blot. Tags group methods across clusters so a technique can be found from more than one route.

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7 articles

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

immunoassays

Comparing ELISA and western blot evidence

ELISA returns a number when the curve holds. A blot returns a band at a mass. Their disagreements point at epitope, matrix, or the hook effect.

8 October 2026 · 10 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

Loading controls are for blots not every gel

Actin, GAPDH and tubulin support a blot loading argument. A purification gel is judged by the band pattern and the total protein you loaded.

8 October 2026 · 6 min read

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

gels and blotting

Loading controls on a western blot

Pick a western loading control for the comparison you are making. A housekeeping band can move, and a total-protein stain is the other argument.

8 October 2026 · 8 min read

Agarose gel with glowing DNA bands on a UV transilluminator under an orange safety shield

gels and blotting

Stripping a blot and why it can fail

Decide whether to strip a western blot or rerun it. A harsh strip can remove the antigen, and a mild strip can leave the first signal behind.

8 October 2026 · 8 min read

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

gels and blotting

Troubleshooting a blank western blot

Reason through a blank western in order: load, transfer, membrane face, antibody, azide, substrate, washes, then a true absence.

8 October 2026 · 8 min read

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

gels and blotting

Western blot from gel to membrane

How a protein moves from an SDS gel onto a membrane, how antibodies create a band, and which controls separate transfer failure from a real signal.

8 October 2026 · 8 min read