protein biochemistry
Codon use and insoluble expression
Why rare codons can slow translation, and why a codon table still does not prove that insoluble expression is a coding problem.
8 October 2026 · 7 min read
Tag
expression
Articles tagged expression. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
7 articles
protein biochemistry
Expression hosts in outline
How to choose E. coli, yeast, insect or mammalian expression from disulfide bonds, glycosylation and the yield the work needs.
8 October 2026 · 8 min read
protein biochemistry
Glycerol stocks of an expression clone
How a clonal glycerol stock fails: thawing the master, satellite colonies, and trusting an antibiotic plate instead of a sequence.
8 October 2026 · 8 min read
protein biochemistry
Inclusion bodies and refolding as a concept
An inclusion body is insoluble expression, not proof a protein can never fold. Refolding is empirical, and activity rather than a band shows success.
8 October 2026 · 7 min read
microbiology
Induction temperature and inclusion bodies
Inclusion bodies are insoluble aggregates from overexpression. A cooler induction can leave more protein soluble, and a gel of soluble and pellet fractions shows which.
8 October 2026 · 10 min read
molecular cloning
Orientation of an insert in an expression vector
Why a backward insert still grows on antibiotic, and how an asymmetric digest, a junction primer or a sequence read shows which way the ORF points.
8 October 2026 · 8 min read
protein biochemistry
Questions for a protein expression enquiry
The sequence, tag, host, modification, purity evidence, endotoxin need and assay buffer a protein expression enquiry has to carry.
8 October 2026 · 9 min read