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Gels and blotting

Agarose and polyacrylamide electrophoresis, transfer methods and how laboratories judge bands, ladders and blot controls.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
western blotantibodysds pageligationelectrophoresisagaroseapparent massdilution seriesdnadna cleanupethidium bromideexposuregel extractionhybridisationloading controloverloadsourcingtransfer

26 articles

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

gels and blotting

Troubleshooting a blank western blot

Reason through a blank western in order: load, transfer, membrane face, antibody, azide, substrate, washes, then a true absence.

8 October 2026 · 8 min read

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

gels and blotting

Western blot from gel to membrane

How a protein moves from an SDS gel onto a membrane, how antibodies create a band, and which controls separate transfer failure from a real signal.

8 October 2026 · 8 min read