protein biochemistry
Loading controls are for blots not every gel
Actin, GAPDH and tubulin support a blot loading argument. A purification gel is judged by the band pattern and the total protein you loaded.
8 October 2026 · 6 min read
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sds page
Articles tagged sds page. Tags group methods across clusters so a technique can be found from more than one route.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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5 articles
Pillar · protein biochemistry
Reading a protein gel
How to read a protein gel: what SDS-PAGE bands, smears, ladders and loading differences can support, and what they cannot identify.
8 October 2026 · 8 min read
protein biochemistry
Reducing agents and disulfide bonds
DTT, beta-mercaptoethanol and TCEP break disulfides in different ways. Reducing and non-reducing gels ask whether oligomers are covalently linked.
8 October 2026 · 8 min read
gels and blotting
SDS-PAGE for protein size estimates
Plan an SDS-PAGE size estimate: stacking and resolving gels, SDS and reduction, apparent mass, and a ladder you do not read past.
8 October 2026 · 9 min read
protein biochemistry
Troubleshooting a smeared protein gel
How to branch a smeared protein gel: overload, salt, DNA, degradation, polymerisation, buffer, bubbles, detergent and a leaking well.
8 October 2026 · 7 min read