gene expression
Troubleshooting flat amplification curves
How to read a flat qPCR trace: empty wells, dead reverse transcriptase, inhibition, seal failure and the wrong channel are different failures.
8 October 2026 · 9 min read
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Gene expression
From RNA quality to RT-qPCR and RNA-seq, including controls, normalisers and the limits of a fold-change claim.
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sequencingrna seqstudy designrt qpcrreportinglibrary prepmetadatano rt controltissuebatch effectsfdrgene expressionnormalisationprimer dimerprimersqpcrreference genesreplicates
26 articles
gene expression
What a differential expression table contains
Which columns in a differential expression table a biologist should question, including fold-change sign, adjusted p and identifier collisions.
8 October 2026 · 9 min read