protein biochemistry
Troubleshooting a smeared protein gel
How to branch a smeared protein gel: overload, salt, DNA, degradation, polymerisation, buffer, bubbles, detergent and a leaking well.
8 October 2026 · 7 min read
Topic cluster
Protein biochemistry
Protein gels, quantification, buffers and purification choices explained for research laboratories.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainspecificationproteomicsenquiryglossarytroubleshootingbuffersquantificationexpressionwestern blotpurificationrecordssds pagealiquotsendotoxinmass spectrometryabsorbanceassays
26 articles
protein biochemistry
What an extinction coefficient assumes
A composition-based extinction coefficient is a conversion with conditions attached. Tags, cofactors and nucleic acid change the number you may report.
8 October 2026 · 6 min read