Skip to content
EVRINTH

Tag

antibodies

Articles tagged antibodies. Tags group methods across clusters so a technique can be found from more than one route.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware

6 articles

Gloved hand holding a stained tissue slide beside a rack of stained microscope slides

immunoassays

Choosing and checking a primary antibody

How to choose and check a primary antibody: host, clonality, application claims, and controls that show the signal is the intended target.

8 October 2026 · 7 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

Pillar · immunoassays

ELISA formats controls and readout

ELISA formats, controls and readout: how direct, sandwich and competitive assays turn a binding event into a number that stays inside the curve.

8 October 2026 · 8 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Questions before an antibody enquiry

Target, species, application, immunogen, host, conjugate and storage on arrival. Each question stops a different wrong reagent from being quoted.

8 October 2026 · 10 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Recombinant versus polyclonal reagents

Polyclonal, hybridoma monoclonal and recombinant antibodies differ in epitope count and lot definition. Choose the class in the specification, not by slogan.

8 October 2026 · 9 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Sodium azide and downstream live-cell work

Sodium azide preserves many antibody stocks, inhibits peroxidase, and poisons cells. Check the vial before an HRP plate or a live culture.

8 October 2026 · 9 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Storage of antibodies after the first thaw

Aliquot before the first thaw, freeze once, and thaw a working tube on ice. The vial sheet sets the temperature. A warm shipment stays quarantined.

8 October 2026 · 11 min read