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EVRINTH

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Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

proteomics

Injection amounts and column loading

Too little peptide yields sparse identifications. Too much broadens peaks, saturates the detector and carries into the next blank. Load follows the column, not a universal volume.

8 October 2026 · 8 min read

Rack of flow cytometry tubes in front of a researcher viewing coloured scatter plots beside a cytometer

cytometry and microscopy

Intracellular staining and Fc block

When to block Fc receptors, and how to order surface stain, fixation and intracellular antibody so sticky cells do not fake a result.

8 October 2026 · 8 min read

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

gene expression

Intron-spanning primers and genomic DNA

How intron-spanning primers make genomic DNA a different product from cDNA, and when a no-RT control is the only honest check.

8 October 2026 · 9 min read

pH electrode in a beaker of buffer while a gloved hand adds drops from a dropper bottle

chromatography

Ion exchange pH and salt

Set ion-exchange pH against the protein charge and plan a salt gradient that binds the target and releases it on purpose.

8 October 2026 · 8 min read

pH electrode in a beaker of buffer while a gloved hand adds drops from a dropper bottle

buffers and water

Ionic strength is not the same as pH

During a hybridisation wash, compute ionic strength from the ions you added. A matched pH can still hide a salt change that moves stringency.

8 October 2026 · 7 min read

Gloved hand weighing white powder with a spatula inside an analytical balance draft shield

liquid handling

ISO 8655 as a calibration framework

Use ISO 8655 as terminology and a user framework, and compare your own gravimetric check with the specification you claim.

8 October 2026 · 7 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Isotype controls are not a full answer

What an isotype control actually measures, which sticky artefacts it can rule out, and why it cannot replace a knockout or a secondary-only well.

8 October 2026 · 8 min read

Gloved hand closing the lid of a benchtop PCR thermal cycler holding a strip of PCR tubes, city lights at dusk behind

labware

Jointed glassware and clamp safety

How to set a standard-taper joint with a PTFE sleeve or grease, a clip and a support, and when a damaged joint leaves service.

8 October 2026 · 8 min read

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

proteomics

Keratin contamination from the bench

Skin, hair, wool and dust shed keratins that can dominate a weak proteomics sample. A blank and the keratin types separate bench contamination from tissue.

8 October 2026 · 9 min read

Wall-mounted water purification unit dispensing ultrapure water into a glass flask

buffers and water

Label expiry is not a stability study

Decide when a printed expiry can be questioned with a passing control, and when sterility, nuclease control, or a calibration standard makes extension the wrong question.

8 October 2026 · 9 min read

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

proteomics

Label-free versus labelled quantification

When a label-free fold change disagrees with a blot, or a labelled ratio looks compressed, check missing values, batch effects, shared peptides and co-isolation.

8 October 2026 · 9 min read