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EVRINTH

Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

520 articles

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

Plant DNA extraction and polyphenols

Plan plant DNA extraction around oxidising polyphenols and co-precipitating polysaccharides, from CTAB-class buffers to young leaf versus woody tissue.

8 October 2026 · 10 min read

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

proteomics

Plasma and the dynamic range problem

Plasma and serum span many orders of magnitude in protein concentration. A plain digest mostly sees albumin and immunoglobulins, and depletion changes what remains.

8 October 2026 · 8 min read

Gloved hand pipetting into a tube near a cold block and agar plates with colonies, gel image on a monitor

Pillar · molecular cloning

Plasmid cloning from insert to colony

How an insert becomes a plasmid, a colony and a verified clone, and which checkpoint fails when the plate is empty or the insert is wrong.

8 October 2026 · 8 min read

Gloved hand pipetting into a tube near a cold block and agar plates with colonies, gel image on a monitor

microbiology

Plasmid stability in a liquid culture

What segregational stability means in liquid culture, and why a cloudy cloning-strain flask can still give you no plasmid.

8 October 2026 · 7 min read

Gloved hand adjusting the stopcock of a resin-packed chromatography column dripping into collection tubes

labware

Plastic resins and solvent compatibility

A working map of polypropylene, polyethylene, polystyrene, polycarbonate, PET and PTFE, and why the solvent chart still decides.

8 October 2026 · 7 min read

Glass beakers and graduated cylinders holding blue and clear liquids on a dark lab bench

labware

Plate colours clear white and black

How to trace a weak or noisy plate reading to well colour: clear for absorbance, white for luminescence, and black for fluorescence.

8 October 2026 · 7 min read

Close-up of a micropipette tip releasing a droplet into a microcentrifuge tube held in a gloved hand

liquid handling

Plate maps so a robot and a person agree

Compare a human plate map with a robot deck map so well identity, A1 orientation, replicates, and controls stay the same.

8 October 2026 · 7 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Plate readers absorbance and fluorescence

Absorbance, fluorescence and luminescence are different reads. A wrong wavelength or a clear plate in a fluorescence assay looks like a failed ELISA.

8 October 2026 · 9 min read

Agarose gel with glowing DNA bands on a UV transilluminator under an orange safety shield

gels and blotting

Ponceau staining before antibody incubation

Stain a blot with Ponceau after transfer and before blocking to see lanes, bubbles and failed transfer. The dye washes off and does not name the antigen.

8 October 2026 · 7 min read

Gloved hand closing the lid of a benchtop PCR thermal cycler holding a strip of PCR tubes, city lights at dusk behind

pcr amplification

Positive negative and no-template controls

Decide when a positive, a negative and a no-template control each earn a place, and when a clean blank still leaves sample lanes unread.

8 October 2026 · 7 min read