protein biochemistry
Enzymes lose activity before they look degraded
An enzyme can lose activity while the Coomassie band remains. Oxidation, carryover, a missing cofactor or partial unfolding need an activity assay.
8 October 2026 · 7 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
cold chain
Enzymes shipped on ice and what to do on arrival
On arrival, name the coolant and move the enzyme to the temperature on its document. A foam shipper is not a place to wait out a meeting.
8 October 2026 · 9 min read
liquid handling
Ergonomics and repetitive pipetting
Plan posture, thumb force, and breaks for a long pipetting session, and report pain as an occupational issue for the institution.
8 October 2026 · 8 min read
labware
Erlenmeyer flasks for culture and mixing
What an Erlenmeyer flask is for in mixing and shake culture, and how baffles, closure and headspace change gas exchange.
8 October 2026 · 7 min read
nucleic acid purification
Ethanol carryover and failed downstream assays
Define wash buffer, residual alcohol, overdrying and elution, then see why leftover ethanol stops a ligation, a PCR or a restriction digest.
8 October 2026 · 7 min read
genome editing
Ethics and approvals are outside a reagent quote
A reagent quotation covers research materials only. Institutional biosafety review, ethics review and germline limits stay with the reader's own institution.
8 October 2026 · 8 min read
research sourcing
Ethics approvals sit outside a product quote
Decide whether human, animal, or clinical-adjacent material needs an institutional ethics or biosafety decision before a kit is quoted.
8 October 2026 · 9 min read
gels and blotting
Ethidium bromide alternatives and still the hazards
Compare ethidium bromide with blue-light and UV nucleic-acid dyes, then match the stain to the imager, the waste rule, and the safety data sheet.
8 October 2026 · 8 min read
laboratory safety
Ethidium bromide and safer dye choices still need care
Treat ethidium bromide as a mutagenic intercalator, and read the SDS for a dye sold as safer. Waste handling stays a local decision.
8 October 2026 · 11 min read
protein biochemistry
Expression hosts in outline
How to choose E. coli, yeast, insect or mammalian expression from disulfide bonds, glycosylation and the yield the work needs.
8 October 2026 · 8 min read
pcr amplification
Extension time and amplicon length
Match extension time to amplicon length, see why a short hold leaves partial products, and why a final extension cannot repair that miss.
8 October 2026 · 8 min read
nucleic acid purification
Extraction controls and blank samples
A reagent blank and a known positive decide whether an extraction batch is interpretable. A contaminated blank stops the whole set.
8 October 2026 · 7 min read