dna sequencing
Controls and spike-ins for a sequencing lane
Which controls belong on a DNA sequencing lane, why an RNA spike-in does not, and what a failed PhiX-style or known-variant control should stop.
8 October 2026 · 9 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware
520 articles
genome editing
Controls for a CRISPR experiment
Place a non-targeting guide, an untransfected well, a known cutter, a second guide and a parental identity check before you treat a fluorescent marker as an edit.
8 October 2026 · 9 min read
cytometry and microscopy
Controls for autofluorescence
How to separate autofluorescence from a real fluorophore using matched unstained cells, the same treatment, and the right detector.
8 October 2026 · 7 min read
research sourcing
Controls the provider and the lab each supply
Separate controls the laboratory must supply from process controls the provider names, and stop the study when either control fails.
8 October 2026 · 8 min read
cell culture
Counting cells and viability dyes
Why a haemocytometer or an automated counter can mislead, and how trypan blue, clumps and debris bias a viability count you will seed from.
8 October 2026 · 8 min read
dna sequencing
Coverage depth is not the same as accuracy
Separate coverage depth from base accuracy, mapping quality, strand bias and PCR copies so a deep pile of one molecule is not truth.
8 October 2026 · 9 min read
labware
Cracked glass and when to retire a vessel
Which rim chips, star cracks and etched graduations mean a glass vessel should be retired, and what to name on the replacement.
8 October 2026 · 7 min read
genome editing
CRISPR interference and activation overview
CRISPRi with dCas9-KRAB and CRISPRa with VP64, VPR or SAM change transcription only while the editor is present. They are not gene knockouts.
8 October 2026 · 10 min read
immunoassays
Cross-reactivity and related proteins
How to check whether an antibody binds a paralogue or an orthologue, using sequence context and a related-protein lane that should stay quiet.
8 October 2026 · 9 min read
laboratory safety
Cryogen burns and oxygen displacement
Cold burns and oxygen displacement are different injuries from one liquid. Compare contact controls with the controls for a small room or a lift.
8 October 2026 · 8 min read
labware
Cryovials and seal design
How internal thread, external thread and an O-ring relate to a manufacturer's limit of nitrogen vapour or liquid-phase storage.
8 October 2026 · 7 min read
cold chain
Cryovials in liquid nitrogen and the explosion risk
A leaking cryovial can fill with liquid nitrogen and burst on warming. Compare that immersion risk with vapour-phase storage before you specify tubes.
8 October 2026 · 9 min read