cytometry and microscopy
Objectives immersion and coverslip thickness
How to match air, water, oil or silicone immersion and the engraved coverslip thickness so a high-NA objective can form a sharp image.
8 October 2026 · 8 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
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520 articles
genome editing
Off-target checks and their limits
In silico lists, GUIDE-seq, CIRCLE-seq, nominated amplicons and genome sequencing each answer a narrower off-target question than the word clean implies.
8 October 2026 · 10 min read
molecular cloning
Orientation of an insert in an expression vector
Why a backward insert still grows on antibiotic, and how an asymmetric digest, a junction primer or a sequence read shows which way the ORF points.
8 October 2026 · 8 min read
molecular cloning
Overlap assembly design rules
How to troubleshoot Gibson-style overlaps of about 15 to 40 bases when repeats, a melting mismatch, a short insert or exonuclease chew-back wrecks the join.
8 October 2026 · 8 min read
research sourcing
Pack size is not the same as the experiment size
Compare the container a seller packs with the amount a design consumes, including dead volume, controls, and one reasonable repeat of the run.
8 October 2026 · 9 min read
genome editing
PAM sequences and why the genome context matters
SpCas9 NGG, SaCas9 NNGRRT and Cas12a TTTV are enzyme motifs beside the spacer. A SNP or closed chromatin can remove a site the reference browser still shows.
8 October 2026 · 7 min read
cytometry and microscopy
Panel design starts with the biology
Which biology, antigen density and spillover rules to name before you assign fluorophores or request a cytometer configuration.
8 October 2026 · 7 min read
cell culture
Passaging before a flask is overgrown
When to split an adherent culture before the flask is crowded, how split ratio and passage number change the next experiment, and what overgrowth does.
8 October 2026 · 9 min read
gene expression
Pathway lists and the multiple-testing problem
How testing thousands of genes creates a multiple-testing problem, and why a pathway table built on an already chosen list is not yet a mechanism.
8 October 2026 · 10 min read
pcr amplification
PCR additives for GC-rich templates
Compare DMSO, betaine, and related additives for GC-rich PCR, including the primer Tm drop and the polymerase inhibition DMSO can cause.
8 October 2026 · 8 min read
pcr amplification
PCR controls and contamination control
How no-template, positive and inhibition controls decide whether a PCR band can be trusted, and how a shared bench keeps old amplicons out of the next setup.
8 October 2026 · 8 min read
pcr amplification
PCR in a teaching laboratory
Plan a teaching PCR on a short amplicon from a safe plasmid, with shared cyclers, one-way gloves and a record the demonstrator keeps.
8 October 2026 · 8 min read