proteomics
TMT and iTRAQ as concepts
TMT and iTRAQ are isobaric reagent classes: one precursor mass, reporter ions for quantity, and ratio compression when other peptides are co-isolated.
8 October 2026 · 8 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
liquid handling
Training a new student without blaming the kit
Define the stops, tip seating, angle, pre-wet, and balance check a new student must show before a reagent kit is blamed.
8 October 2026 · 7 min read
laboratory safety
Training records and who may use a cabinet
A certified cabinet is not a trained user. The record should show who was shown the airflow, the sash height, and the signs of failure.
8 October 2026 · 8 min read
gels and blotting
Transfer buffers and membrane choice
Compare wet, semi-dry and rapid transfer, then match nitrocellulose or PVDF, methanol and pore size to the protein you must keep.
8 October 2026 · 9 min read
buffers and water
Tris buffers and temperature
Compare Tris with HEPES by the temperature of use. Tris pH falls as it warms; specify that temperature before you raise a reagent enquiry.
8 October 2026 · 8 min read
gels and blotting
Troubleshooting a blank western blot
Reason through a blank western in order: load, transfer, membrane face, antibody, azide, substrate, washes, then a true absence.
8 October 2026 · 8 min read
cytometry and microscopy
Troubleshooting a cytometer with no events
What no events means on a cytometer: clog, threshold, trigger, sheath, interlock, or a tube that is not actually sipping.
8 October 2026 · 7 min read
cytometry and microscopy
Troubleshooting a dim microscope image
How to check shutter, filter cube, objective, correction collar, focus and exposure before calling a dim field a negative result.
8 October 2026 · 7 min read
liquid handling
Troubleshooting a dripping pipette
Name the seal, shaft, solvent, and piston causes of a dripping pipette, and keep that pipette out of service until it is checked.
8 October 2026 · 6 min read
dna sequencing
Troubleshooting a failed library quantification
A library number is not a molarity until the assay is named. How dye, adapter qPCR and a fragment trace disagree, and which disagreement matters.
8 October 2026 · 7 min read
chromatography
Troubleshooting a flat chromatogram
Work through a flat chromatogram by checking the lamp, wavelength, injection, leak, flow-through, and the integrator scale.
8 October 2026 · 7 min read
immunoassays
Troubleshooting a high background
High background means the blank is loud. Separate a dirty blank from a plate that is loud in every well before you subtract and move on.
8 October 2026 · 9 min read