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EVRINTH

Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles

Gloved hand closing the lid of a benchtop PCR thermal cycler holding a strip of PCR tubes, city lights at dusk behind

gene expression

Standard curves and primer efficiency

How a standard curve turns primer slope into efficiency, and how R-squared, dynamic range, no-template controls and primer dimers decide if the line is usable.

8 October 2026 · 10 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Standard curves that bend

Why immunoassay standard curves are sigmoid, where the usable window sits, and what an early bend or a forced straight line does to a reported value.

8 October 2026 · 9 min read

Gloved hand placing a vial into the autosampler of a modular HPLC system with solvent bottles on top

chromatography

Standards and retention time windows

Set a retention window from a standard on this method, and widen or stop when temperature, column age, or mobile phase moves it.

8 October 2026 · 7 min read

Erlenmeyer flasks of amber bacterial culture clamped on an orbital shaker platform

microbiology

Sterile technique for cloning strains

How aseptic handling of non-pathogenic E. coli cloning strains keeps dust, loops and open lids from turning a plate into a mixed culture.

8 October 2026 · 11 min read

Glass beakers and graduated cylinders holding blue and clear liquids on a dark lab bench

labware

Sterile versus clean is not the same

How to specify sterile, clean, endotoxin-controlled or nuclease-controlled labware as separate claims with different evidence.

8 October 2026 · 7 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Storage of antibodies after the first thaw

Aliquot before the first thaw, freeze once, and thaw a working tube on ice. The vial sheet sets the temperature. A warm shipment stays quarantined.

8 October 2026 · 11 min read

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

Storing eluted DNA and RNA

DNA is more forgiving than RNA once eluted. Choose TE or water, plan aliquots and freeze-thaw, and treat humidity and power cuts as storage risks.

8 October 2026 · 8 min read

Researcher viewing gene expression heatmaps and genomic tracks on two monitors at night

gene expression

Stranded libraries and transcript orientation

How stranded RNA-seq libraries keep transcript direction, and why antisense or overlapping genes are unreadable when the chemistry is unstranded.

8 October 2026 · 9 min read